Anti-Cyclin B1/CCNB1 Antibody Picoband®

CCNB also known as Cyclin B1, is a protein that in humans is encoded by the CCNB1 gene, and it is mapped to 5q13.2. The protein encoded by this gene is a regulatory protein involved in mitosis. The gene product complexes with p34 (cdc2) to form the maturation-promoting factor (MPF) . Two alternative transcripts have been found, a constitutively expressed transcript and a cell cycle-regulated transcript, that is expressed predominantly during G2/M phase. The different transcripts result from the use of alternate transcription initiation sites. CCNB contributes to the switch-like all or none behavior of the cell in deciding to commit to mitosis. Its activation is well-regulated, and positive feedback loops ensure that once the cyclin B1-Cdk1 complex is activated, it is not deactivated.

E.coli-derived human Cyclin B1 recombinant protein (Position: M1-V433) . Human Cyclin B1 shares 86% and 85% amino acid (aa) sequences identity with mouse and rat Cyclin B1, respectively.

1. Sartor H, Ehlert F, Grzeschik KH, Muller R, Adolph S (Aug 1992) . Assignment of two human cell cycle genes, CDC25C and CCNB1, to 5q31 and 5q12, respectively. Genomics 13 (3) : 911–2. 2. Kimura K, Hirano M, Kobayashi R, Hirano T (October 1998) . Phosphorylation and activation of 13S condensin by Cdc2 in vitro. Science 282 (5388) : 487–90. 3. Heald R, McKeon F (May 1990) . Mutations of phosphorylation sites in lamin A that prevent nuclear lamina disassembly in mitosis. Cell 61 (4) : 579–89.

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.