Anti-Crk p38 Antibody Picoband®

CRK, also known as p38 or CRKII, is a protein that in humans is encoded by the CRK gene. This gene is a member of an adapter protein family that binds to several tyrosine-phosphorylated proteins. It is mapped to 17p13.3. The CRK protein participates in the Reelin signaling cascade downstream of DAB1. The product of this gene has several SH2 and SH3 domains (src-homology domains) and is involved in several signaling pathways, recruiting cytoplasmic proteins in the vicinity of tyrosine kinase through SH2-phosphotyrosine interaction. The N-terminal SH2 domain of this protein functions as a positive regulator of transformation whereas the C-terminal SH3 domain functions as a negative regulator of transformation. Two alternative transcripts encoding different isoforms with distinct biological activity have been described.

E.coli-derived human Crk p38 recombinant protein (Position: A2-R246) . Human Crk p38 shares 99% and 100% amino acid (aa) sequences identity with mouse and rat Crk p38, respectively.

The Crk-I and Crk-II forms differ in their biological activities. Crk-II has less transforming activity than Crk-I. Crk- II mediates attachment-induced MAPK8 activation, membrane ruffling and cell motility in a Rac-dependent manner. Involved in phagocytosis of apoptotic cells and cell motility via its interaction with DOCK1 and DOCK4. May regulate the EFNA5-EPHA3 signaling. .

1. Mayer BJ, Hanafusa H (1990) . Association of the v-crk oncogene product with phosphotyrosine-containing proteins and protein kinase activity.. Proc Natl Acad Sci U S A 87 (7) : 2638–42. 2. Matsuki T, Pramatarova A, Howell BW (June 2008) . Reduction of Crk and CrkL expression blocks reelin-induced dendritogenesis. J. Cell. Sci. 121 (Pt 11) : 1869–75. 3. Park TJ, Curran T (December 2008) . Crk and Crk-like play essential overlapping roles downstream of disabled-1 in the Reelin pathway. J. Neurosci. 28 (50) : 13551–62.

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.