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Anti-Collagen IV/COL4A1 Antibody Picoband®

Boster Bio Anti-Collagen IV/COL4A1 Antibody Picoband® catalog # PB9099. Tested in IF, IHC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Product Specifications

Background

COL4A1, also known as ICH or Collagen alpha-1 (IV), is a protein that in humans is encoded by the COL4A1 gene. It is mapped to 13q34. This gene encodes the major type IV alpha collagen chain of basement membranes. Like the other members of the type IV collagen gene family, this gene is organized in a head-to-head conformation with another type IV collagen gene so that each gene pair shares a common promoter. COL4A1 binds to alpha-1/beta-1 integrin and inhibits migration, proliferation, and tube formation by endothelial cells. It is also a potential therapeutic candidate for targeting tumor angiogenesis.

Synonyms

Collagen alpha-1 (IV) chain; Arresten; COL4A1

Gene Name

COL4A1

Gene ID

1282

UniProt

P02462

Host

Rabbit

Reactivity

Human

Cross Reactivity

No cross-reactivity with other proteins

Immunogen

E.coli-derived human Collagen IV recombinant protein (Position: G1445-T1669) . Human Collagen IV shares 97% amino acid (aa) sequence identity with mouse Collagen IV.

Clonality

Polyclonal

Tissue Specificity

Highly expressed in placenta.

Applications

IF, IHC, WB

Field of Research

Collagen, Cytoskeleton/ECM, ECM Proteins, Extracellular Matrix, Mesenchymal Stem Cells, Signal Transduction, Stem Cells

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

Type IV collagen is the major structural component of glomerular basement membranes (GBM), forming a 'chicken-wire' meshwork together with laminins, proteoglycans and entactin/nidogen. Arresten, comprising the C-terminal NC1 domain, inhibits angiogenesis and tumor formation. The C-terminal half is found to possess the anti-angiogenic activity. Specifically inhibits endothelial cell proliferation, migration and tube formation. Inhibits expression of hypoxia-inducible factor 1alpha and ERK1/2 and p38 MAPK activation. Ligand for alpha1/beta1 integrin.

References & Citations

1. Soininen R, Haka-Risku T, Prockop DJ, Tryggvason K (Jan 1988) . Complete primary structure of the alpha 1-chain of human basement membrane (type IV) collagen. FEBS Lett 225 (1-2) : 188–94. 2. Plaisier E, Gribouval O, Alamowitch S et al. (2007) . COL4A1 mutations and hereditary angiopathy, nephropathy, aneurysms, and muscle cramps. N. Engl. J. Med. 357 (26) : 2687–2695. 3. Wang, X., Harris, R. E., Bayston, L. J., Ashe, H. L. Type IV collagens regulate BMP signalling in Drosophila. Nature 455: 72-77, 2008.

Storage Conditions

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

138564 MW

Observed Molecular Weight

250 kDa

Specificity

No cross reactivity with other proteins.

Gene Name Synonym

Collagen, type IV, alpha 1

Subcellular Location

Basement membrane.

Sequence Similarities

Belongs to the type IV collagen family.

Protein Name

Collagen alpha-1 (IV) chain

Isotype

Rabbit IgG

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

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