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EF-2 (phospho Thr56) Polyclonal Antibody

Rabbit Anti-EF-2 (phospho Thr56) Polyclonal Antibody

Product Specifications

Background

EEF2 encodes a member of the GTP-binding translation elongation factor family. Elongation factor 2 is an essential factor for protein synthesis. It promotes the GTP-dependent translocation of the nascent protein chain from the A-site to the P-site of the ribosome. This protein is completely inactivated by EF-2 kinase phosporylation.

Product Name Alternative

EEF2; EF2; Elongation factor 2; EF-2

Gene ID

1938

Accession Number

P13639

Reactivity

Human, Mouse, Rat

Immunogen

Synthesized peptide derived from human EF-2 around the phosphorylation site of T56

Clonality

Polyclonal

Tag

Elongation factor 2

Applications

WB, IHC, IF, ELISA

Purification Method

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration

1 mg/ml

Molecular Weight

100kD

Precautions

The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Shipping Conditions

Gel pack with blue ice.

Storage Conditions

Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Notes

Phospho-EF-2 (T56) Polyclonal Antibody detects endogenous levels of EF-2 protein only when phosphorylated at T56.

Product Datasheet

https://www.abbkine.com/datasheet/ABP0151.pdf

Formulation

Liquid solution

Buffer pH

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Applications Notes

Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000; IHC 1:100-1:300; IF 1:200-1:1000; ELISA 1:10000; Not yet tested in other applications.

Other Product Names

EEF2; EF2; Elongation factor 2; EF-2

Tested Applications

WB, IHC, IF, IP, ELISA

Host or Source

Rabbit

Accession Number URL

http://www.uniprot.org/uniprot/P13639

Isotype

Rabbit IgG

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