PCNA Polyclonal Antibody

Rabbit Anti-PCNA Polyclonal Antibody

Product Specifications

Background

Proliferating cell nuclear antigen encoded by PCNA is found in the nucleus and is a cofactor of DNA polymerase delta. Proliferating cell nuclear antigen acts as a homotrimer and helps increase the processivity of leading strand synthesis during DNA replication. In response to DNA damage, proliferating cell nuclear antigen is ubiquitinated and is involved in the RAD6-dependent DNA repair pathway. Two transcript variants encoding the same protein have been found for PCNA. Pseudogenes of PCNA have been described on chromosome 4 and on the X chromosome.

Product Name Alternative

PCNA; Proliferating cell nuclear antigen; PCNA; Cyclin

Gene ID

5111

Accession Number

P12004

Reactivity

Human, Mouse, Rat, Monkey, Rabbit

Immunogen

Synthesized peptide derived from the Internal region of human PCNA at AA range: 30-110

Clonality

Polyclonal

Tag

Proliferating cell nuclear antigen

Applications

WB, IHC, IF, ELISA

Purification Method

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration

1 mg/ml

Molecular Weight

30-33kD

Precautions

The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Shipping Conditions

Gel pack with blue ice.

Storage Conditions

Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Notes

PCNA Polyclonal Antibody detects endogenous levels of PCNA protein.

Formulation

Liquid solution

Buffer pH

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Applications Notes

Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-2000; IHC 1:100-500; IF/ICC1:100-500; ELISA 1:5000-20000