Anti-Caspase-9 (I20) Antibody

Rabbit Polyclonal Antibody specific to Caspase-9 (I120)

Product Specifications

CAS Number

9007-83-4

Product Name Alternative

CASP-9, EC 3.4.22.62, Apoptotic protease Mch-6, Apoptotic protease-activating factor 3, APAF-3, ICE-like apoptotic protease 6, ICE-LAP6 [Cleaved into: Caspase-9 subunit p35; Caspase-9 subunit p10]

Gene Name

CASP9

Gene ID

842

NCBI Gene ID

UniProt

P55211

Accession Number

NP_001220

Host

Rabbit

Reactivity

Human

Immunogen

Peptide corresponding to aa 299- 318 of human caspase-9 (accession no. P55211).

Target Antigen

Caspase-9

Target

Caspase-9 (I20)

Clonality

Polyclonal

Isotype

IgG

Type

Antibody

Applications

ELISA, WB, ICC, IF, IP

Field of Research

Apoptosis

Purification Method

Purified by peptide immuno-affinity chromatography

Concentration

Lot Specific

Dilution

Dilute in PBS or medium which is identical to that used in the assay system.

Format

Purified

Form

Liquid

Buffer

Phosphate Buffered Saline

Function

Involved in the activation cascade of caspases responsible for apoptosis execution. Binding of caspase-9 to Apaf-1 leads to activation of the protease which then cleaves and activates caspase-3. Promotes DNA damage-induced apoptosis in a ABL1/c-Abl-dependent manner. Proteolytically cleaves poly(ADP-ribose) polymerase (PARP).; Isoform 2 lacks activity is an dominant-negative inhibitor of caspase-9.

Additionnal Information

Immunoblotting: use at 1ug/mL. Immunocytochemistry: use at 2ug/mL. These are recommended concentrations. Enduser should determine optimal concentrations for their applications. Positive control: Whole cell lysate from HeLa cells.

Storage Conditions

This antibody is stable for at least one (1) year at -20°C. Avoid multiple freeze-thaw cycles.

Specificity

This antibody recognizes full-length human caspase-9 (46kDa).

Formulation

PBS, pH 7.4.

Buffer pH

pH 7.4

Target Background

Cell death signals are transduced by death domain-containing adapter molecules and members of the caspase family of proteases. Caspase-9 and Apaf-1 bind to each other, which leads to caspase-9 activation. Caspase-9 is also activated by granzyme B and CPP32. Activated caspase-9 cleaves and activates caspase-3, a protease responsible for proteolytic cleavage of many key proteins involved in apoptosis. Caspase-9 plays a central role in cell death induced by a wide variety of apoptosis induced by a wide variety of apoptosis inducers including TNF , TRAIL, anti-CD95, FADD, and TRADD. Caspase-9 is expressed in a variety of human tissues.