Keratinocytes Basal Medium, 500 ml

Keratinocyte Basal Medium serum-freeBio&SELL Keratinocyte Medium is a ready-to-use medium for serum-free cultivation of human keratinocytes.The basal medium is MCDB medium. After supplementation, ready-to-use medium for human keratinocytes.With the keratinocyte medium serum-free, the frequent excessive growth of fibroblasts is suprimed, resulting in selectively better growth of human keratinocytes. This medium must be supplemented before use.Here's how to easily supplement your medium:Thaw supplements at room temperature.Add supplements to the basal medium (ensure sterility) .Trypsinise keratinocytes, wash with PBS and stop remaining trypsin with trypsin inhibitor (10 mg/ml) .Perform another washing step (PBS) and then determine cell count. In case of subcultivation, adjust cell density to 3000 to 5000 cells/ml. (Initial culture 1 - 5 x 106/cells/cm2) . Incubate keratinocytes serum-free with supplemented keratinocyte medium in the incubator.Alternatively, coat culture dishes with attachment factors (collagen, fibronectin or natural or synthetic fragments of these factors) beforehand.