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Cell-Free Protein Synthesis Kit Max (Lyophilized)

The Cell-Free Protein Synthesis Kit is a product for in vitro protein synthesis based on E. coli lysate. Coupled transcription/translation systems contain an RNA polymerase and the necessary cellular machinery needed to direct protein synthesis (e.g., ribosomes, translation factors and tRNAs) . Supplements such as amino acids, an energy source and NTPs complete the system. Proteins are expressed using DNA or RNA as templates. Cell-free protein expression, independent of viable cells, enables rapid, flexible and high-yield protein expression, with numerous advantages over traditional recombinant expression systems: 1. Time saving. Express target proteins in 1~2 hours, with peak yield achieved in 8~24 hours. 2. High protein expression level, up to more than 3 mg/ml. 3. Easy operation, Add DNA or RNA templates to the reaction system, and the reaction can be performed using 96-well plates or centrifuge tubes. Note: The product is suitable for expressing diverse proteins, particularly disulfide-rich proteins.

Product Specifications

Assay Protocol

http://en.best-enzymes.com/storage/upload/files/directions/11danbaiyanjiuxilie/EG25304.pdf

Components

• Cell-free system solution A Max (Lyophilized) : 1 vial (300 μl after reconstitution) • Cell-free system solution B (Lyophilized) : 1 vial (600 μl after reconstitution) • CFPS-Control Plasmid: 2 μg

Shipping Conditions

2~8°C

Storage Conditions

4°C transportation. Store at -20°C. Valid for 12 months. After reconstitution, store at -20°C for no more than 1 month, and at -80°C for no more than 12 months.

Shelf Life

1 Year

Frequently Asked Questions

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