T4 Polynucleotide Kinase

T4 Polynucleotide Kinase (T4 PNK) is a polyribonucleotide 5'-hydroxyl kinase that catalyzes the transfer of the γ-phosphate group from ATP to the 5'-hydroxyl terminus of oligonucleotide chains, whether they are double-stranded or single-stranded DNA or RNA. Notably, this reaction is reversible. In addition, T4 PNK exhibits 3'-phosphatase activity, which hydrolyzes the 3'-phosphate group from the 3'-phosphate terminus of oligonucleotides, deoxy-3'-monophosphate nucleotides, and deoxy-3'-diphosphate nucleotides.