Exonuclease I

Exonuclease I (E. coli) is a single-strand specific exonuclease that degrades single-stranded DNA in the 3'-5' direction, releasing 5'-deoxyribonucleotides. Exo I exhibits strong specificity for single-stranded DNA and does not degrade double-stranded DNA or RNA. Additionally, it cannot degrade single-stranded DNA with 3'-OH terminiblocked by phosphoryl or acetyl groups. This product is obtained by recombinant expression of the Exo I gene (E. coli) in Escherichia coli, followed by multiple purification steps.