Thermostable RNase T1

Thermostable RNase T1 is a thermostable mutant derived from RNase T1 through directed protein evolution. Compared to the wild-type RNase T1, Thermostable RNase T1 retains 100% of its activity at 50°C, while the activity of the wild-type decreases by at least 50% at the same temperature. The higher reaction temperature facilitates the complete unfolding of RNA secondary structures, reducing or avoiding their interference with the enzymatic cleavage process, thereby making the digestion more thorough.