RNase T1

RNase T1 is an endoribonuclease obtained from Aspergillus oryzae, which exhibits specificity for cleaving single-stranded RNA following guanosine ribonucleotides (G), resulting in the formation of 3'-phosphate termini. RNase T1 is capable of forming a nucleoside 2', 3'-cyclic phosphate intermediate to cleave the phosphodiester bond between the 3'-guanosine residue and the 5'-OH group of the adjacent nucleoside, producing oligonucleotides with terminal 3'-GMP and 3'-GMP. The activity of RNase T1 does not depend on metal ions.