DNase I, RNase-free

DNase I, RNase-free is an endodeoxyribonuclease that catalyzes to the same extent the degradation of both single- and double-stranded DNA randomly, and produces 5'-P terminal oligonucleotides. The activity of DNase I is dependent on Ca 2+ , and can be activated by bivalent metal ions Mg 2+ , Mn 2+ , etc. In the presence of Mg 2+ , the enzyme can randomly recognize and cut any site on any strand of double-stranded DNA. In the presence of Mn 2+ , nearly identical sites on the two strands of DNA can be recognized and cut, resulting in flat ends or sticky end DNA fragments with 1 to 2 nucleotides overhang.