BbvCI

BbvCI is derived from the BbvCI gene of Bacillus brevis (L. Ge) and is obtained through recombinant expression in Escherichia coli. BbvCI is a Type IIA restriction enzyme existing as a heterodimer composed of two distinct catalytic subunits, each with an independent cleavage site. It recognizes and cleaves the non-palindromic sequence CCTCAGC (-5/-2), generating 5' cohesive ends with 3-base overhangs. BbvCI uses CutOne® reaction buffer and is compatible with double digestion with other LightNing® restriction enzymes. However, it is advisable to avoid extended digestion (exceeding 3 hours) to prevent star activity.