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LightNing® PvuI

LightNing® enzymes are a series of engineered restriction enzymes that are capable of fast DNA digestion. All LightNing® enzymes show superior activity in the universal CutOne® and CutOne® Color Buffer, and are able to digest DNA in 5~15 minutes. This enables any combination of restriction enzymes to work simultaneously in one reaction tube and eliminates the need for sequential digestions. LightNing® enzymes have passed multiple strict quality controls, and can be used to digest plasmid, genomic and viral DNA as well as PCR products. CutOne® Color Buffer includes a density reagent along with red and yellow tracking dyes that allow for direct loading of the reaction mixtures on a gel. The red dye of the CutOne® Color Buffer migrates with 2.5 kb double-strand DNA fragments in a 1% agarose gel, and the yellow dye migrates with 10 bp double-strand DNA fragments in a 1% agarose gel.

Product Specifications

Assay Protocol

http://en.best-enzymes.com/storage/upload/files/directions/04xianzhimei/EG23413.pdf

Concentration

20 U/μL

Components

• LightNing® PvuI (20 U/μl) : 30 μl (600 U) • 10x CutOne® Buffer: 1 ml • 10x CutOne® Color Buffer: 1 ml

Features & Benefits

The reaction can be completed within 15 minutes Recommended Reaction Conditions 1x CutOne® Buffer; Incubate at 37°C; Refer to "Protocol for Fast DNA Digestion" for reaction setup. Heat Inactivation Incubation at 80°C for 20 minutes. Methylation sensitivity Cleavage with this restriction enzyme may be blocked or impaired when the substrate DNA is methylated by the CpG methylase.

Shipping Conditions

2~8°C

Storage Conditions

-20°C

Shelf Life

2 Years

Isoschizomers

Ple19I

Cut Site

CGATCG 5'...C G A T ↓ C G...3' 3'...G C ↑ T A G C...5'

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