Welcome to GenPrice! Check out our latest updates.

Shopping Cart (0)

Your cart is empty

Add some products to get started!

LightNing® PspGI (EcoRII)

LightNing® enzymes are a series of engineered restriction enzymes that are capable of fast DNA digestion. All LightNing® enzymes show superior activity in the universal CutOne® and CutOne® Color Buffer, and are able to digest DNA in 5~15 minutes. This enables any combination of restriction enzymes to work simultaneously in one reaction tube and eliminates the need for sequential digestions. LightNing® enzymes have passed multiple strict quality controls, and can be used to digest plasmid, genomic and viral DNA as well as PCR products. CutOne® Color Buffer includes a density reagent along with red and yellow tracking dyes that allow for direct loading of the reaction mixtures on a gel. The red dye of the CutOne® Color Buffer migrates with 2.5 kb double-strand DNA fragments in a 1% agarose gel, and the yellow dye migrates with 10 bp double-strand DNA fragments in a 1% agarose gel.

Product Specifications

Assay Protocol

http://en.best-enzymes.com/storage/upload/files/directions/04xianzhimei/15560.pdf

Concentration

10 U/μL

Components

• LightNing® PspGI (10 U/μl) : 200 μl (2000 U) • 10x CutOne® Buffer: 2x1 ml • 10x CutOne® Color Buffer: 2x1 ml

Features & Benefits

The reaction can be completed within 15 minutes Recommended Reaction Conditions 1x CutOne® Buffer; Incubate at 75°C; Refer to "Protocol for Fast DNA Digestion" for reaction setup. Heat Inactivation This enzyme can not be heat inactivated. Please purify the digested product by phenol/chloroform treatment or column-based purification kit. Methylation sensitivity Cleavage with this restriction enzyme may be blocked or impaired when the substrate DNA is methylated by the Dcm methylase.

Shipping Conditions

2~8°C

Storage Conditions

-20°C

Shelf Life

2 Years

Isoschizomers

EcoRII, Psp6I, AjnI

Cut Site

CCWGG 5'... ↓ C C W G G ...3' 3'... G G W C C ↑ ...5'

More Discoveries

Explore Other Products

Browse additional items from our catalog

ProSAAS (PCSK1N) (NM_013271) Human Mass Spec Standard
PH301065 10 µg

ProSAAS (PCSK1N) (NM_013271) Human Mass Spec Standard

Sign In for Pricing
View Details
Recombinant Cyanidium caldarium Photosystem II reaction center psb28 protein (psb28)
MBS1464590-01 0.02 mg (E-Coli)

Recombinant Cyanidium caldarium Photosystem II reaction center psb28 protein (psb28)

Sign In for Pricing
View Details
Recombinant Cyanidium caldarium Photosystem II reaction center psb28 protein (psb28)
MBS1464590-02 0.1 mg (E-Coli)

Recombinant Cyanidium caldarium Photosystem II reaction center psb28 protein (psb28)

Sign In for Pricing
View Details
Recombinant Cyanidium caldarium Photosystem II reaction center psb28 protein (psb28)
MBS1464590-03 0.02 mg (Yeast)

Recombinant Cyanidium caldarium Photosystem II reaction center psb28 protein (psb28)

Sign In for Pricing
View Details
Recombinant Cyanidium caldarium Photosystem II reaction center psb28 protein (psb28)
MBS1464590-04 0.1 mg (Yeast)

Recombinant Cyanidium caldarium Photosystem II reaction center psb28 protein (psb28)

Sign In for Pricing
View Details
Recombinant Cyanidium caldarium Photosystem II reaction center psb28 protein (psb28)
MBS1464590-05 0.02 mg (Baculovirus)

Recombinant Cyanidium caldarium Photosystem II reaction center psb28 protein (psb28)

Sign In for Pricing
View Details