Welcome to GenPrice! Check out our latest updates.

Shopping Cart (0)

Your cart is empty

Add some products to get started!

LightNing® DraIII

LightNing® enzymes are a series of engineered restriction enzymes that are capable of fast DNA digestion. All LightNing® enzymes show superior activity in the universal CutOne® and CutOne® Color Buffer, and are able to digest DNA in 5~15 minutes. This enables any combination of restriction enzymes to work simultaneously in one reaction tube and eliminates the need for sequential digestions. LightNing® enzymes have passed multiple strict quality controls, and can be used to digest plasmid, genomic and viral DNA as well as PCR products. CutOne® Color Buffer includes a density reagent along with red and yellow tracking dyes that allow for direct loading of the reaction mixtures on a gel. The red dye of the CutOne® Color Buffer migrates with 2.5 kb double-strand DNA fragments in a 1% agarose gel, and the yellow dye migrates with 10 bp double-strand DNA fragments in a 1% agarose gel.

Product Specifications

Assay Protocol

http://en.best-enzymes.com/storage/upload/files/directions/04xianzhimei/EG24508-1.pdf

Concentration

20 U/μL

Components

• LightNing® DraIII (20 U/μl) : 30 μl (600 U) • 10x CutOne® Buffer: 1 ml • 10x CutOne® Color Buffer: 1 ml

Features & Benefits

The reaction can be completed within 15 minutes Recommended Reaction Conditions 1x CutOne® Buffer; Incubate at 37°C; Refer to "Protocol for Fast DNA Digestion" for reaction setup. Heat Inactivation This enzyme can not be heat inactivated. Please purify the digested product by phenol/chloroform treatment or column-based purification kit. Methylation sensitivity Cleavage with this restriction enzyme may be blocked or impaired when the substrate DNA is methylated by the CpG methylase. Cleavage with this restriction enzyme may be blocked or impaired when the substrate DNA is methylated by the EcoKI methylase. Cleavage with this restriction enzyme may be blocked or impaired when the substrate DNA is methylated by the EcoBI methylase.

Shipping Conditions

2~8°C

Storage Conditions

-20°C

Shelf Life

2 Years

Isoschizomers

AdeI

Cut Site

CACNNNGTG 5'...C A C N N N ↓ G T G...3' 3'...G T G ↑ N N N C A C...5'

Frequently Asked Questions

More Discoveries

Explore Other Products

Browse additional items from our catalog

Protein Kinase C (19-31) 10mg
A23135-10 10 mg

Protein Kinase C (19-31) 10mg

Sign In for Pricing
View Details
Human BDNF (Brain Derived Neurotrophic Factor) ELISA Kit
E39EH0043 96 Tests

Human BDNF (Brain Derived Neurotrophic Factor) ELISA Kit

Sign In for Pricing
View Details
6-Chloro-4-hydroxy-7-methyl-2-oxo-2H-chromene-3-carbaldehyde ≥97% (HPLC)
234632-01 100 mg

6-Chloro-4-hydroxy-7-methyl-2-oxo-2H-chromene-3-carbaldehyde ≥97% (HPLC)

Sign In for Pricing
View Details
6-Chloro-4-hydroxy-7-methyl-2-oxo-2H-chromene-3-carbaldehyde ≥97% (HPLC)
234632-02 250 mg

6-Chloro-4-hydroxy-7-methyl-2-oxo-2H-chromene-3-carbaldehyde ≥97% (HPLC)

Sign In for Pricing
View Details
6-Chloro-4-hydroxy-7-methyl-2-oxo-2H-chromene-3-carbaldehyde ≥97% (HPLC)
234632-03 1 g

6-Chloro-4-hydroxy-7-methyl-2-oxo-2H-chromene-3-carbaldehyde ≥97% (HPLC)

Sign In for Pricing
View Details
6-Chloro-4-hydroxy-7-methyl-2-oxo-2H-chromene-3-carbaldehyde ≥97% (HPLC)
234632-04 5 g

6-Chloro-4-hydroxy-7-methyl-2-oxo-2H-chromene-3-carbaldehyde ≥97% (HPLC)

Sign In for Pricing
View Details