M-MLV GIII Reverse Transcriptase
M-MLV GIII Reverse Transcriptase is third-generation reverse transcriptase derived through genetic engineering and recombinant technology from the Moloney Murine Leukemia Virus (M-MLV) . This enzyme has been modified to eliminate the RNase H activity. Additionally, it exhibits significantly improved thermal stability (optimal reaction temperature:50 °C) . These greatly improve the specificity and length of the synthesized first-strand cDNA, allowing for the synthesis of cDNA fragments up to 12 kb. Moreover, M-MLV GIII Reverse Transcriptase shows enhanced tolerance to RNA secondary structures.
Product Specifications
Assay Protocol
http://en.best-enzymes.com/storage/upload/files/directions/02nizhuanlu/EG15124.pdf
Concentration
200 U/μL
Components
• M-MLV GIII Reverse Transcriptase (200 U/μl) : 50 μl • 5x M-MLV First Strand Buffer: 500 μl • 0.1 M DTT: 100 μl
Shipping Conditions
2~8°C
Storage Conditions
Store at -20°C for 2 years.
Shelf Life
2 Years
Frequently Asked Questions
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