2x Taq PCR Aurora Mix

Taq PCR Aurora Mix is a ready-to-use PCR mix with the concentration of 2x. lt offers convenience and reduces the risk of contamination. PCR products synthesized with this mix have an overhang of A bases at the 3' end, enabling direct T/A cloning after purification. The Mix contains Taq DNA polymerase and a protein with 3'→5' exonuclease activity, enabling efficient amplification of DNA fragments up to 7 kb with high yields. Coupled with the optimized reaction buffer, it can achieve efficient amplification for different GC contents (ranging from 30% to 70%) . In addition, compared with WT-Taq DNA polymerase, the extension rate of Taq PCR Aurora Mix is enhanced by 2 to 4 times, which can effectively shorten the reaction time. This PCR Mix contains two dyes. The PCR products can be directly loaded for electrophoresis without adding a Loading Buffer, and two indicator bands in blue and red colors will appear during the electrophoresis process. These dyes do not interfere with the efficiency of PCR amplification. However, for experiments that require optical analyses such as absorbance and fluorescence of the PCR products, it is recommended to purify the PCR products prior to analysis.