Phi29 DNA Polymerase
Phi29 DNA Polymerase is a DNA polymerase cloned from Bacillus subtilis phage phi29. phi29 DNA Polymerase exhibits a strong strand-displacement activity, a strong chain affinity, and a single polymerization can achieve a continuous polymerization extension up to 70 kb. In addition, phi29 DNA Polymerase has extremely high fidelity due to its inherent 3'→5' exonuclease activity. However, because of the enzymes's 3'→5' proofreading exonuclease, 3'-modified primers are highly recommended.
Product Specifications
Assay Protocol
http://en.best-enzymes.com/storage/upload/files/directions/01juhemei/EG25102.pdf
Concentration
10 U/μL
Components
• phi29 DNA Polymerase (10 U/μl) : 5x50 μl • 10x phi29 Buffer: 2x1 ml
Shipping Conditions
2~8°C
Storage Conditions
Store at -20°C for 2 years.
Shelf Life
2 Years
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