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Bst II DNA Polymerase (Large Fragment)

Bst II DNA Polymerase (Large Fragment) is a modified form of Bst DNA polymerase obtained by truncating the 5' → 3' exonuclease domain. Through directed evolution and recombinant expression in Escherichia coli , this enzyme has been engineered. It possesses robust 5' → 3' DNA polymerase activity and strand displacement activity. The optimal reaction temperature for this enzyme is 65°C, and it is suitable for various isothermal amplification reactions.Compared to the wild-type enzyme, Bst II DNA Polymerase (Large Fragment) exhibits faster amplification rates, enhanced tolerance to dUTP and salt, as well as improved stability.

Product Specifications

Assay Protocol

http://en.best-enzymes.com/storage/upload/files/directions/01juhemei/EG23101-1.pdf

Concentration

8 U/μL

Components

• Bst II DNA Polymerase (Large Fragment) (8 U/μl) : 1 ml • 10x Bst II Reaction Buffer: 3x1 ml • MgSO4 (100 mM) : 3x1 ml

Shipping Conditions

2~8°C

Storage Conditions

-20°C

Shelf Life

2 Years

Frequently Asked Questions

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