Bst II DNA Polymerase (Large Fragment)
Bst II DNA Polymerase (Large Fragment) is a modified form of Bst DNA polymerase obtained by truncating the 5' → 3' exonuclease domain. Through directed evolution and recombinant expression in Escherichia coli , this enzyme has been engineered. It possesses robust 5' → 3' DNA polymerase activity and strand displacement activity. The optimal reaction temperature for this enzyme is 65°C, and it is suitable for various isothermal amplification reactions.Compared to the wild-type enzyme, Bst II DNA Polymerase (Large Fragment) exhibits faster amplification rates, enhanced tolerance to dUTP and salt, as well as improved stability.
Product Specifications
Assay Protocol
http://en.best-enzymes.com/storage/upload/files/directions/01juhemei/EG23101-1.pdf
Concentration
8 U/μL
Components
• Bst II DNA Polymerase (Large Fragment) (8 U/μl) : 1 ml • 10x Bst II Reaction Buffer: 3x1 ml • MgSO4 (100 mM) : 3x1 ml
Shipping Conditions
2~8°C
Storage Conditions
-20°C
Shelf Life
2 Years
Frequently Asked Questions
Explore Other Products
Browse additional items from our catalog
Catenin, beta (CTNNB1) (CTNNB1/2098), CF594 conjugate, 0.1mg/mL
