SpCas9 D10A Nickase

SpCas9 D10A Nickase is a mutant of the Cas9 protein. SpCas9 D10A Nickase retains the function of a cleavage domain of Cas9 nuclease and specifically cleaves the target single strand to form a nick. SpCas9 D10A Nickase reduces off-target effects[1].

Product Specifications

UNSPSC

12352204

Target

Biochemical Assay Reagents; CRISPR/Cas9

Type

Enzyme

Related Pathways

Cell Cycle/DNA Damage; Others

Field of Research

Others

Assay Protocol

https://www.medchemexpress.com/spcas9-d10a-nickase.html

Solubility

10 mM in DMSO

Smiles

[SpCas9D10ANickase]

References & Citations

[1]Jasper Che-Yung Chien, et al. Multiplexed bioluminescence-mediated tracking of DNA double-strand break repairs in vitro and in vivo. Nat Protoc. 2021, 16, 8.