Anti-GluA1/GluR1 Glutamate Receptor (N355/1) Recombinant Chicken Chimeric mAb

Our chicken Anti-GluA1/GluR1 glutamate receptor recombinant monoclonal antibody is a chimeric antibody derived from NeuroMab clone N355/1. It detects human, mouse, and rat GluA1/GluR1 is purified by affinity chromatography. It works in WB, IHC, ICC, ELISA.

Product Specifications

Background

Glutamate receptor 1 or GluA1/GluR1 glutamate receptor is a member of the glutamate-gated ion channel family. GluR1 funcitons as an excitatory neurotransmitter at many synapses in the central nervous system and is widely expressed in brain. Mutations in the GluR1 gene are associated with cerebral cortical dysplasia, Status Epilepticus, and depression.

Short Description

Our chicken Anti-GluA1/GluR1 glutamate receptor recombinant monoclonal antibody is a chimeric antibody derived from NeuroMab clone N355/1. It detects human, mouse, and rat GluA1/GluR1 glutamate receptor and is purified by affinity chromatography. It works in WB, IHC, ICC, ELISA.

Synonyms

Glutamate receptor 1 (GluR-1) (AMPA-selective glutamate receptor 1) (GluR-A) (GluR-K1) (Glutamate receptor ionotropic, AMPA 1) (GluA1)

Gene Name

Gria1 Glur1

Host

Chicken

Reactivity

Human, Mouse, and Rat

Immunogen

Fusion protein amino acids 1-389 (extracellular N-terminus) of rat GluA1/GluR1 (accession number P19490) produced recombinantly in E. Coli.

Target

GluA1/GluR1 glutamate receptor

Clonality

Recombinant

Clone

N355/1

Conjugation

Unconjugated

Applications

WB, IHC, ICC, ELISA

Field of Research

/ /Neurotransmitter Receptors

Assay Type

Primary Antibodies

Concentration

1 mg/mL

Format

Purified by affinity chromatography

Form

Liquid

pH

7.4

Molecular Weight

100 kDa

Precautions

These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.

Shipping Conditions

Shipped on ice packs

Storage Conditions

Aliquot and store at ≤ -20°C for long term storage. For short term storage, store at 2-8°C. For maximum recovery of product, centrifuge the vial prior to removing the cap.

Notes

This recombinant antibody is a chimeric antibody created by replacing the mouse heavy and light constant regions of clone N355/1 with chicken IgY heavy and light constant regions. As such this antibody retains the same binding performance as the original clone N355/1 but can be detected using standard anti-chicken secondary antibodies allowing flexibility for multiplexing applications. This antibody is expressed recombinantly in mammalian cells and then affinity purified from the cell culture media.

IHC Dilution

1:250-1:1000

WB Dilution

1:500-1:1000

Specificity

Does not cross-react with GluR2

ICC Dilution

1:250-1:1000

Quality Control

Each new lot of antibody is quality control tested by Western blot on rat brain membrane fraction and confirmed to stain the expected molecular weight band.