Anti-GluN1/NR1 Glutamate Receptor (N308/48) Recombinant Chicken Chimeric mAb

Our chicken Anti-GluN1/NR1 glutamate receptor recombinant monoclonal antibody is a chimeric antibody derived from NeuroMab clone N308/48. It detects human, mouse, and rat GluN1/NR1 is purified by affinity chromatography. It works in WB, IHC, ICC, ELISA.

Product Specifications

Background

Glutamate receptor 1, NMDA 1, or GluN1/NR1 also known as subunit 1 of the N-methyl-D-aspartate (NMDA) receptor. It is a member of the glutamate receptor channel superfamily and is a heteromeric glutamate-gated calcium ion channel. GluN1 is essential for synaptic function in the brain. GluN1 is expressed throughout the brain in cerebellum, thalamus, olfactory bulb and cortex in the membrane at postsynaptic density. GluN1 plays a key role in synaptogenesis, synaptic plasticity, memory acquisition and learning. Mutations in the GluN1 gene are associated with intellectual disability and cortical visual impairment and movement disorders.

Short Description

Our chicken Anti-GluN1/NR1 glutamate receptor recombinant monoclonal antibody is a chimeric antibody derived from NeuroMab clone N308/48. It detects human, mouse, and rat GluN1/NR1 glutamate receptor and is purified by affinity chromatography. It works in WB, IHC, ICC, ELISA.

Synonyms

Glutamate receptor ionotropic, NMDA 1 (GluN1) (Glutamate [NMDA] receptor subunit zeta-1) (N-methyl-D-aspartate receptor subunit NR1) (NMD-R1)

Gene Name

Grin1 Nmdar1

Host

Chicken

Reactivity

Human, Mouse, and Rat

Immunogen

Fusion protein amino acids 42-361 (extracellular N-terminus) of rat NR1 (accession number P35439) produced recombinantly in E. Coli

Target

GluN1/NR1 glutamate receptor

Clonality

Recombinant

Clone

N308/48

Conjugation

Unconjugated

Applications

WB, IHC, ICC, ELISA

Field of Research

/ /Neurotransmitter Receptors

Assay Type

Primary Antibodies

Concentration

1 mg/mL

Format

Purified by affinity chromatography

Form

Liquid

pH

7.4

Molecular Weight

105 kDa

Precautions

These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.

Shipping Conditions

Shipped on ice packs

Storage Conditions

Aliquot and store at ≤ -20°C for long term storage. For short term storage, store at 2-8°C. For maximum recovery of product, centrifuge the vial prior to removing the cap.

Notes

This recombinant antibody is a chimeric antibody created by replacing the mouse heavy and light constant regions of clone N308/48 with chicken IgY heavy and light constant regions. As such this antibody retains the same binding performance as the original clone N308/48 but can be detected using standard anti-chicken secondary antibodies allowing flexibility for multiplexing applications. This antibody is expressed recombinantly in mammalian cells and then affinity purified from the cell culture media.

IHC Dilution

1:500-1:1000

WB Dilution

1:500-1:1000

Specificity

No cross-reactivity reported

ICC Dilution

1:250-1:500

Quality Control

Each new lot of antibody is quality control tested by Western blot on rat brain membrane fraction and confirmed to stain the expected molecular weight band.