Anti-Aldh1L1 (N103/39) Recombinant Chicken Chimeric mAb

Our chicken Anti-Aldh1L1 recombinant monoclonal antibody is a chimeric antibody derived from NeuroMab clone N103/39. It detects human, mouse, non-human primate, and rat Aldh1L1, and is purified by affinity chromatography. It is great for use in ELISA, ICC, IHC, WB.

Product Specifications

Background

Aldehyde dehydrogenase family 1 member L1 (Aldh1L1) is a member of the aldehyde dehydrogenase family. This protein is an enzyme that catalyzes the conversion of 10-formyltetrahydrofolate, NADP, and water to tetrahydrofolate, NADPH, and carbon dioxide. It is an astrocyte marker and also is expressed in the liver. It is proposed to play a role in regulation of cellular THF levels and inhibiting cell proliferation. Alterations in Aldh1L1 have been implicated in Congenital Hydrocephalus

Short Description

Synonyms

Cytosolic 10-formyltetrahydrofolate dehydrogenase (10-FTHFDH) (FDH) (EC 1.5.1.6) (Aldehyde dehydrogenase family 1 member L1) (FBP-CI)

Gene Name

Aldh1l1 Fthfd

Host

Chicken

Reactivity

Human, Mouse, Non-Human Primate, and Rat

Immunogen

Protein amino acids 1-902 (full-length) of rat Aldh1L1 (accession number P28037) produced recombinantly in E. Coli

Target

Aldh1L1

Clonality

Recombinant

Clone

N103/39

Conjugation

Unconjugated

Applications

ELISA, ICC, IHC, WB

Field of Research

Cell Markers/Glial and Schwann Cell Markers/Astrocyte Markers

Assay Type

Primary Antibodies

Concentration

1 mg/mL

Format

Purified by affinity chromatography

Form

Liquid

pH

7.4

Molecular Weight

100 kDa

Precautions

These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.

Shipping Conditions

Shipped on ice packs

Storage Conditions

Aliquot and store at ≤ -20°C for long term storage. For short term storage, store at 2-8°C. For maximum recovery of product, centrifuge the vial prior to removing the cap.

Notes

This recombinant antibody is a chimeric antibody created by replacing the mouse heavy and light constant regions clone N103/39 with chicken IgY heavy and light constant regions. As such this antibody retains the same binding performance as the original clone N103/39 but can be detected using standard anti-chicken secondary antibodies allowing flexibility for multiplexing applications. This antibody is expressed recombinantly in mammalian cells and then affinity purified from the cell culture media.

IHC Dilution

1:250-1:1000

WB Dilution

1:1000-1:2000

Specificity

No cross-reactivity against Aldh1L2

ICC Dilution

1:1000

Quality Control

Each new lot of antibody is quality control tested by Western blot on rat whole brain lysate and confirmed to stain the expected molecular weight band.