Anti-RBPMS (OTI3B7-GP) Recombinant Guinea Pig Chimeric mAb

Guinea pig monoclonal recombinant chimeric RBPMS antibody from clone OTI3B7. Validated in WB, IHC, ICC, flow cytometry and purified by Protein A chromatography.

Product Specifications

Background

RBPMS (RNA binding protein with multiple splicing), also known as HERMES, contains one RRM (RNA recognition motif) domain and belongs to the RRM family of RNA-binding proteins. RBPMS exists as multiple alternatively spliced isoforms and is thought to bind RNA, possibly playing a role in RNA-related events, such as transcription and translation. RNA-binding proteins that are specific to retinal ganglion cells (RGCs) have been previously identified as excellent markers for RGCs (Kwong et al., 2010) . Recent findings show that antibodies against RBPMS are robust reagents that exclusively identify RGCs in multiple mammalian species (Rodriguez et al. 2014) .

Short Description

This guinea pig RBPMS recombinant monoclonal antibody takes the place of our 1832-RBPMS guinea pig polyclonal antibody. It is a chimeric antibody, combining the antigen-binding variable region of mouse clone OTI3B7 with the constant region of the guinea pig IgG molecule, making it perfect for reproducible multiplexing. It detects human, mouse, and rat RBPMS and works in WB, IHC, ICC, and flow cytometry.

Synonyms

FLJ32971 antibody, Heart and RRM expressed sequence antibody, Hermes antibody, RBP MS antibody, RBP-MS antibody, Rbpms antibody, RBPMS_HUMAN antibody, RNA binding protein gene with multiple splicing antibody, RNA-binding protein with multiple splicing antibody

Gene Name

RBPMS

NCBI Gene ID

19663

Host

Guinea Pig

Reactivity

Human, Mouse, and Rat

Immunogen

Full length human recombinant protein of human RBPMS (NP_001008712) produced in E.coli.

Target

RBPMS

Clonality

Recombinant

Isotype

IgG

Clone

OTI3B7-GP

Conjugation

Unconjugated

Applications

WB, IHC, ICC, Flow cytometry

Field of Research

Quality Control: WB/Cell Markers/ Retinal Cell Markers/ Retinal Ganglion Cell Markers

Assay Type

Primary Antibodies

Concentration

1 mg/mL

Format

Purified by Protein A Chromatography

Form

Liquid

pH

7.4

Molecular Weight

24 kDa

Precautions

These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.

Shipping Conditions

Shipped on ice packs

Storage Conditions

Aliquot and store at ≤ -20°C for long term storage. For short term storage, store at 2-8°C. For maximum recovery of product, centrifuge the vial prior to removing the cap.

Notes

This recombinant antibody is a chimeric antibody created by replacing the mouse heavy and light constant regions of clone OTI3B7 with guinea pig IgG heavy and light constant regions. As such this antibody retains the same binding performance as the original clone OTI3B7 but can be detected using standard anti-guinea pig secondary antibodies allowing flexibility for multiplexing applications. This antibody is expressed recombinantly in mammalian cells and then affinity purified from the cell culture media.

IHC Dilution

1:250-1:2500

WB Dilution

1:1000-1:5000

Specificity

Specific for endogenous levels of the ~24 kDa RBPMS protein.

ICC Dilution

1:1000

Quality Control

Each new lot of antibody is quality control tested by Western blot on rat heart lysate and confirmed to stain the expected molecular weight band.

Uniprot Accession Number

Q93062

Immunogen Species

Human

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