CD40L/CD154/TRAP, Rat (HEK293, Fc)

CD40L (CD154; TRAP) is a ligand to CD40/TNFRSF5, acts function by generating a costimulatory signal that up-regulates IL-4 synthesis[1]. CD40L is specifically expressed on activated CD4+ T-lymphocytes and involves in activation of NF-κB/MAPK pathway[2][3]. CD40L also involves in B cell differentiation, maturation, and apoptosis[4]. CD40L in rat, cleaved into 2 chains of membrane form (1-260 a.a.) and soluble form (112-260 a.a.) which serves as a ligand for integrins (ITGA5:ITGB1 and ITGAV:ITGB3) . CD40L/CD154/TRAP Protein, Rat (HEK293, Fc) has a total length of 146 amino acids (G115-L260), is expressed in HEK392 cells with N-terminal hFc-tag.

Product Specifications

Product Name Alternative

CD40L/CD154/TRAP Protein, Rat (HEK293, Fc), Rat, HEK293

UNSPSC

12352202

Molecular Formula

84349 (Gene_ID) Q9Z2V2/NP_445805.1 (G115-L260) (Accession)

Molecular Weight

Approximately 53 kDa, based on SDS-PAGE under reducing conditions, due to the glycosylation.

References & Citations

[1]Blotta MH, et al. Cross-linking of the CD40 ligand on human CD4+ T lymphocytes generates a costimulatory signal that up-regulates IL-4 synthesis. J Immunol. 1996 May 1;156 (9) :3133-40.|[2]Schwabe RF, et al. CD40 activates NF-kappa B and c-Jun N-terminal kinase and enhances chemokine secretion on activated human hepatic stellate cells. J Immunol. 2001 Jun 1;166 (11) :6812-9.|[3]Batlle A, et al. CD40 and B-cell receptor signalling induce MAPK family members that can either induce or repress Bcl-6 expression. Mol Immunol. 2009 May;46 (8-9) :1727-35.|[4]Mikolajczak SA, et al. The modulation of CD40 ligand signaling by transmembrane CD28 splice variant in human T cells. J Exp Med. 2004 Apr 5;199 (7) :1025-31.|[5]Takada YK, et al. Soluble CD40L activates soluble and cell-surface integrin αvβ3, α5β1, and α4β1 by binding to the allosteric ligand-binding site (site 2) . J Biol Chem. 2021 Jan-Jun;296:100399.|[6]Pietravalle F, et al. Human native soluble CD40L is a biologically active trimer, processed inside microsomes. J Biol Chem. 1996 Mar 15;271 (11) :5965-7.