PNGase F (Immobilized, Microspin)
PNGase F (Immobilized, Microspin) is a resin in which the PNGase F (peptide N-glycosidase F) enzyme is covalently coupled to agarose beads for the removal of N-glycans from antibodies, fusion proteins, and other N-glycosylated proteins. The enzyme is recombinantly expressed in E.coli and the sequence is derived from Flavobacterium meningsepticum[1][2].
Product Specifications
CAS Number
[83534-39-8]
UNSPSC
12352204
Target
Glycosidase
Related Pathways
Metabolic Enzyme/Protease
Field of Research
Cancer
Purity
0
Smiles
[PNGaseF(Immobilized,Microspin)]
References & Citations
[1]Vilaj M, et al. Evaluation of different PNGase F enzymes in immunoglobulin G and total plasma N-glycans analysis. Glycobiology. 2021 Jan 9;31 (1) :2-7. |[2]Huang J, et al. Highly Efficient Release of Glycopeptides from Hydrazide Beads by Hydroxylamine Assisted PNGase F Deglycosylation for N-Glycoproteome Analysis. Anal Chem. 2015 Oct 20;87 (20) :10199-204.
Shipping Conditions
Room Temperature
Storage Conditions
Store at 4°C for 2 years
Scientific Category
Enzyme
Clinical Information
No Development Reported
Curated Selection
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