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Hydroxyphenyl Fluorescein (solution)

Hydroxyphenyl Fluorescein (HPF) (solution) is a stable ROS fluorescent probe dye. Hydroxyphenyl Fluorescein has stronger specificity and stability than H2DCFDA. Hydroxyphenyl Fluorescein can produce strong green fluorescence through hydroxyl radical reaction with intracellular peroxynitroso. Hydroxyphenyl Fluorescein can be applied for fluorescence microscopy, high-throughput imager, luciferase microplate reader or flow cytometry. Ex/Em=490/515 nm[1].Solvent and concentration: DMSO: 10 mM

Product Specifications

CAS Number

359010-69-8

Target

Fluorescent Dye; Reactive Oxygen Species (ROS)

Related Pathways

Immunology/Inflammation; Metabolic Enzyme/Protease; NF-κB; Others

Field of Research

Others

Smiles

OC1=CC=C(OC2=CC3=C(C4(C5=CC=C(C=C5O3)O)C6=C(C(O4)=O)C=CC=C6)C=C2)C=C1

Molecular Formula

C26H16O6

Molecular Weight

424.41

References & Citations

[1]Ming-De Li, et al. Dynamics of Oxygen-Independent Photocleavage of Blebbistatin as a One-Photon Blue or Two-Photon Near-Infrared Light-Gated Hydroxyl Radical Photocage. J Am Chem Soc. 2018 Nov 21;140 (46) :15957-15968.

Shipping Conditions

Room temperature

Scientific Category

Dye Reagents

Clinical Information

No Development Reported

Available Sizes

Frequently Asked Questions

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