Propidium (Iodide) (solution)

Propidium Iodide (PI) (solution) is a nuclear staining agent that stains DNA. Propidium Iodide is an analogue of ethidine bromide that emits red fluorescence upon embedding in double-stranded DNA. Propidium Iodide cannot pass through living cell membranes, but it can pass through damaged cell membranes to stain the nucleus. Propidium Iodide has a fluorescence wavelength of 493/617 nm and a wavelength of 536/635 nm after Mosaic with DNA. Propidium Iodide is commonly used in the detection of apoptosis (apoptosis) or necrosis (necrosis), and is often used in flow cytometry analysis.Solvent and Concentration: Sterile water: 1 mg/mL

Product Specifications

CAS Number

[25535-16-4]

Target

DNA/RNA Synthesis; Fluorescent Dye

Related Pathways

Cell Cycle/DNA Damage; Others

Field of Research

Others

Smiles

C[N+](CCC[N+]1=C(C2=CC=CC=C2)C3=CC(N)=CC=C3C4=C1C=C(N)C=C4)(CC)CC.[I-].[I-]

Molecular Formula

C27H34I2N4

Molecular Weight

668.41

References & Citations

[1]Hezel M, et al. Propidium iodide staining: a new application in fluorescence microscopy for analysis of cytoarchitecture in adult and developing rodent brain. Micron. 2012 Oct;43 (10) :1031-8.|[2]A rapid and simple method for measuring thymocyte apoptosis by propidium iodidestaining and flow cytometry. J Immunol Methods. 1991 Jun 3;139 (2) :271-9.|[3]Nicoletti I, et al. A rapid and simple method for measuring thymocyte apoptosis by propidium iodide staining and flow cytometry. J Immunol Methods. 1991 Jun 3;139 (2) :271-9.