DAPI
DAPI (4',6-Diamidino-2-phenylindole) is a DAPI dye. DAPI is a fluorescent dye that binds strongly to DNA. It binds to the AT base pair of the double-stranded DNA minor groove, and one DAPI molecule can occupy three base pair positions. The fluorescence intensity of DAPI molecules bound to double-stranded DNA is increased by about 20 times, and it is commonly observed with fluorescence microscopy, and the amount of DNA can be determined based on the intensity of fluorescence. In addition, because DAPI can pass through intact cell membranes, it can be used to stain both live and fixed cells[1]. DAPI (Compound 3) is an acid-sensing ion channel 3 (ASIC3) inhibitor. DAPI binds to ASIC3 and blocks the channel function. DAPI can be used in the study of chronic pain treatment[2] (Ex/Em = 356/451 nm) .
Product Specifications
CAS Number
[47165-04-8]
Product Name Alternative
4',6-Diamidino-2-phenylindole
UNSPSC
12171500
Target
DNA Stain; Sodium Channel
Related Pathways
Cell Cycle/DNA Damage; Membrane Transporter/Ion Channel
Field of Research
Neurological Disease
Purity
98.01
Solubility
DMSO : 9.09 mg/mL (ultrasonic; adjust pH to 3 with 1 M HCL)
Smiles
N=C(C1=CC=C(C2=CC3=CC=C(C(N)=N)C=C3N2)C=C1)N
Molecular Formula
C16H15N5
Molecular Weight
277.32
References & Citations
[1]Kuduk SD, et al. Amidine derived inhibitors of acid-sensing ion channel-3 (ASIC3) . Bioorg Med Chem Lett. 2009 Aug 1;19 (15) :4059-63.|[2]Kapuscinski J, et al. DAPI: a DNA-specific fluorescent probe. Biotech Histochem. 1995 Sep;70 (5) :220-33.
Shipping Conditions
Blue Ice
Storage Conditions
-20°C (Powder, protect from light)
Scientific Category
Dye Reagents
Clinical Information
No Development Reported
Citation 01
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