Tetrazolium Red

Tetrazolium Red (2,3,5-Triphenyltetrazolium chloride; TTC) is a not brain-penetrant, colorless, water-soluble dye that is reduced by mitochondrial enzymes to a deep red, water-insoluble compound (formazan) mainly in the mitochondria of living cells. Tetrazolium Red is used to observe the activity of dehydrogenase, and it turns colorless to red when exposed to hydrogen. Tetrazolium Red distinguishes between surviving and infarcted brain tissue after stroke. Tetrazolium Red has been used to stain heart tissue to measure the extent of acute lesions and also used to stain brain tissue to detect the size of the infarcted area. The absorption wavelength of Tetrazolium Red is 570 nm[1][2][3][4].

Product Specifications

CAS Number

[298-96-4]

Product Name Alternative

2,3,5-Triphenyltetrazolium chloride; TPTZ; TTC

UNSPSC

12352200

Hazard Statement

H228, H315, H319

Target

Biochemical Assay Reagents

Related Pathways

Others

Field of Research

Cardiovascular Disease

Purity

99.93

Solubility

DMSO : 16.67 mg/mL (ultrasonic) |H2O : 50 mg/mL (ultrasonic)

Smiles

C1(C2=CC=CC=C2)=NN(C3=CC=CC=C3)[N+](C4=CC=CC=C4)=N1.[Cl-]

Molecular Formula

C19H15ClN4

Molecular Weight

334.80

Precautions

H228, H315, H319

References & Citations

[1]Sun YY, et al. Mannitol-facilitated perfusion staining with 2,3,5-triphenyltetrazolium chloride (TTC) for detection of experimental cerebral infarction and biochemical analysis. J Neurosci Methods. 2012 Jan 15;203 (1) :122-9.|[2]Bederson JB, et al. Evaluation of 2,3,5-triphenyltetrazolium chloride as a stain for detection and quantification of experimental cerebral infarction in rats. Stroke. 1986 Nov-Dec;17 (6) :1304-8.|[3]Lin YC, et al. Human umbilical mesenchymal stem cells promote recovery after ischemic stroke. Stroke. 2011 Jul;42 (7) :2045-53.|[4]Sylvester PW. Optimization of the tetrazolium dye (MTT) colorimetric assay for cellular growth and viability. Methods Mol Biol. 2011;716:157-68.