IZ medium serum-free, 500 ml

Immune cell medium IZ serum-freeBio&SELL's IZ medium is an optimised serum-free medium for the effective cultivation and propagation of human immune cells (T, NK, CIK and LAK cells) in vitro - "ready-to-use"!For you, "ready-to-use" means:No expensive supplementation (with serum) No need for time-consuming serum testsNo batch to batch variationsSignificantly lower risk of contaminationTime savingReliable, absolutely reproducible resultsComposition of the IZ medium:The basis for the IZ medium is the RPMI 1640 and the DMEM-F12 medium, which have been enriched by the addition of special salts, trace elements, albumin, cholesterol, lipids and vitamins. The IZ medium deliberately does not contain any growth factors (such as recombinant cytokines) for cell differentiation or the enrichment of T cells from whole blood samples. These can thus be added in accordance with individual requirements.Fields of application:Proliferation of T cells from peripheral blood mononuclear cells (PBMC) /whole blood after stimulation.Proliferation of NK, LAK and CIK cells after stimulationLong-term cultivation of immortalised T cellsCell culture of immune cellsCells of the peripheral blood are arrested in the G0 phase and must be stimulated with mitogens (plant lectins, e.g. phytohemaggluthinin PHA; or antibodies such as anti-CD3) in order to be cultivated for some time in the cell culture.Tips for stimulating T cells:T-cell density of 1x105/ mlAdd PHA according to the manufacturer's instructions (1-5 μg/ml) .Incubation time is between 48-72 hrs (depending on the type and origin of the lymphocytes and their use) Addition of fresh IZ medium and further stimulation of the T cells is necessary to continue the culturePlease note: the proliferation rate of primary lymphocytes from whole blood is limitedTips for stimulation / cultivation of CIKCIK are enriched by adding IFN-γ, mAb anti-CD3 (OKT3) and IL-2 to isolated PBMCs (peripheral blood mononuclear cells) . After 2-3 weeks in vitro culture, their concentration is increased 1000-fold.