2-5A

2-5A is a 5'-triphosphorylated (2',5') oligoadenylate. 2-5A is an immunotransmitter that fuels RNase L immunity. 2-5A degrades viral mRNA and inhibits protein synthesis by binding to RNase L and activating its endoribonuclease activity. 2-5A can be used in RSV and cancer research[1][2][3][4].

Product Specifications

CAS Number

[65954-93-0]

Product Name Alternative

2′,5′-ApApA; 2′,5′-trioligoadenylate; 5'-O-Triphosphoryladenylyl- (2'→5') -adenylyl- (2'→5') -adenosine

UNSPSC

12352005

Target

DNA/RNA Synthesis; RSV

Related Pathways

Anti-infection; Cell Cycle/DNA Damage

Applications

COVID-19-anti-virus

Field of Research

Cancer; Infection; Inflammation/Immunology

Smiles

NC1=NC=NC2=C1N=CN2[C@H]3[C@H](OP(O)(OC[C@@H](O[C@H]([C@@H]4OP(O)(OC[C@@H](O[C@H]([C@@H]5O)N6C=NC7=C6N=CN=C7N)[C@H]5O)=O)N8C=NC9=C8N=CN=C9N)[C@H]4O)=O)[C@H](O)[C@@H](COP(OP(OP(O)(O)=O)(O)=O)(O)=O)O3

Molecular Formula

C30H40N15O25P5

Molecular Weight

1165.59

References & Citations

[1]Kalinichenko EN, et al. 3'-Fluoro-3'-deoxy analogs of 2-5A 5'-monophosphate: binding to 2-5A-dependent endoribonuclease and susceptibility to (2'-5') phosphodiesterase degradation. Biochem Biophys Res Commun. 1990 Feb 28;167 (1) :20-6.|[2]Barnard DL, Sidwell RW, Xiao W, Player MR, Adah SA, Torrence PF. 2-5A-DNA conjugate inhibition of respiratory syncytial virus replication: effects of oligonucleotide structure modifications and RNA target site selection. Antiviral Res. 1999 Apr;41 (3) :119-34. |[3]Wang S, Li L. 2-5A is an immunotransmitter that fuels RNase L immunity. Immunity. 2025 Apr 8;58 (4) :770-772. |[4]Huai W, et al. OAS cross-activates RNase L intercellularly through cell-to-cell transfer of 2-5A to spread innate immunity. Immunity. 2025 Apr 8;58 (4) :797-810.e6. doi: 10.1016/j.immuni.2025.01.016. Epub 2025 Feb 25.