Nitro blue tetrazolium chloride
Nitro blue tetrazolium chloride (NBT) is a substrate for dehydrogenases; is used with the alkaline phosphatase substrate 5-Bromo-4-Chloro-3-Indolyl Phosphate (BCIP) in western blotting and immunohistological staining procedures[1].
Product Specifications
CAS Number
[298-83-9]
Product Name Alternative
NBT
UNSPSC
12171500
Hazard Statement
H302, H315, H319, H335
Target
Fluorescent Dye
Related Pathways
Others
Field of Research
Others
Concentration
10mM
Purity
99.82
Solubility
70% DMF in Water : 100 mg/mL (ultrasonic) |DMSO : 5 mg/mL (ultrasonic; warming; heat to 60°C) |H2O : 8 mg/mL (ultrasonic)
Smiles
COC1=CC(C2=CC=C(N3N=C(C4=CC=CC=C4)N=[N+]3C5=CC=C([N+]([O-])=O)C=C5)C(OC)=C2)=CC=C1N6N=C(C7=CC=CC=C7)N=[N+]6C8=CC=C([N+]([O-])=O)C=C8.[Cl-].[Cl-]
Molecular Formula
C40H30Cl2N10O6
Molecular Weight
817.64
Precautions
H302, H315, H319, H335
References & Citations
[1]Trinh le A, et al. Fluorescent in situ hybridization employing the conventional NBT/BCIP chromogenic stain. Biotechniques. 2007 Jun;42 (6) :756-9.
Shipping Conditions
Room Temperature
Storage Conditions
4°C (Powder, sealed storage, away from moisture and light)
Scientific Category
Dye Reagents
Clinical Information
No Development Reported
Available Sizes
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