RWJ-60475

RWJ-60475 is a cell-permeable tyrosine phosphatase inhibitor with an IC50 of 3.3 μM. RWJ-60475, by inhibiting the activity of CD45/CD148 phosphatase, interferes with the phagocytic function of macrophages, thereby blocking the invasion of Legionella pneumophila into host cells and the transport of effector proteins. RWJ-60475 significantly reduces the uptake of bacterial particles by macrophages. RWJ-60475 can be used in the research of anti-infection targeting host factors[1][2].

Product Specifications

CAS Number

[204130-08-5]

UNSPSC

12352005

Target

Bacterial; Phosphatase

Related Pathways

Anti-infection; Metabolic Enzyme/Protease

Applications

COVID-19-immunoregulation

Field of Research

Infection

Smiles

OP(O)(C(C1=CC([N+]([O-])=O)=CC=C1OC2=CC=C(Br)C=C2)O)=O

Molecular Formula

C13H11BrNO7P

Molecular Weight

404.11

References & Citations

[1]Charpentier X, et al. Chemical genetics reveals bacterial and host cell functions critical for type IV effector translocation by Legionella pneumophila. PLoS pathogens. 2009 Jul 3;5 (7) :e1000501.|[2]Karvinen J, et al. Fluorescence quenching-based assays for hydrolyzing enzymes. Application of time-resolved fluorometry in assays for caspase, helicase, and phosphatase. Anal Chem. 2004 Mar 1;76 (5) :1429-36.