(Rac) -Z-FA-FMK

(Rac) -Z-FA-FMK is the racemate of Z-FA-FMK. (Rac) -Z-FA-FMK is an inhibitor of cathepsin B with a Ki of 1.5 μM. (Rac) -Z-FA-FMK inhibits caspase-2, -3, -6, -7, and -9 with IC50s of 6.147, 15.41, 32.45, 9.077, and 110.7 μM. (Rac) -Z-FA-FMK inhibits the main protease of SARS-CoV-2 replication with an IC50 of 11.39 μM. (Rac) -Z-FA-FMK inhibits the increased IL-1β level induced by LPS and NF-κB transactivation in macrophages[1][2][3][4].

Product Specifications

CAS Number

[96922-64-4]

UNSPSC

12352005

Target

Caspase; Cathepsin; Interleukin Related; NF-κB; SARS-CoV

Related Pathways

Anti-infection; Apoptosis; Immunology/Inflammation; Metabolic Enzyme/Protease; NF-κB

Field of Research

Infection

Smiles

O=C(NC(CC1=CC=CC=C1)C(NC(C)C(CF)=O)=O)OCC2=CC=CC=C2

Molecular Formula

C21H23FN2O4

Molecular Weight

386.42

References & Citations

[1]Smith, R. A., et al., (1988) . Inhibition of cathepsin B by peptidyl aldehydes and ketones: slow-binding behavior of a trifluoromethyl ketone. Biochemistry, 27 (17), 6568–6573. |[2]Lopez-Hernandez, F. J., et al., (2003) . Z-FA-fmk inhibits effector caspases but not initiator caspases 8 and 10, and demonstrates that novel anticancer retinoid-related molecules induce apoptosis via the intrinsic pathway. Molecular cancer therapeutics, 2 (3), 255–263. |[3]Zhu, W., et al., (2020) . Identification of SARS-CoV-2 3CL Protease Inhibitors by a Quantitative High-Throughput Screening. ACS pharmacology & translational science, 3 (5), 1008–1016.|[4]Schotte, P., et al., (2001) . The cathepsin B inhibitor z-FA.fmk inhibits cytokine production in macrophages stimulated by lipopolysaccharide. The Journal of biological chemistry, 276 (24), 21153–21157.