Hippuryl-His-Leu-OH

Hippuryl-His-Leu-OH (N-Benzoyl-Gly-His-Leu) is a substrate for detecting the activity of angiotensin I converting enzyme. The His-Leu released by Hippuryl-His-Leu-OH can react with o-phtbaldialdehyde or Fluorescamine (HY-D0715) for fluorescence detection[1][2][3].

Product Specifications

CAS Number

[31373-65-6]

Product Name Alternative

N-Benzoyl-Gly-His-Leu

UNSPSC

12352209

Target

Amino Acid Derivatives; Angiotensin-converting Enzyme (ACE)

Type

Peptides

Related Pathways

Metabolic Enzyme/Protease; Others

Applications

Metabolism-protein/nucleotide metabolism

Field of Research

Cardiovascular Disease

Assay Protocol

https://www.medchemexpress.com/hippuryl-his-leu-oh.html

Solubility

10 mM in DMSO

Smiles

CC(C)C[C@@H](C(O)=O)NC([C@@H](NC(CNC(C1=CC=CC=C1)=O)=O)CC2=CN=CN2)=O

Molecular Formula

C21H27N5O5

Molecular Weight

429.47

References & Citations

[1]Nishimura K, et al. Purification of angiotensin I-converting enzyme from human lung. Biochim Biophys Acta. 1977 Aug 11;483 (2) :398-408. |[2]Santos RA, et al. An improved fluorometric assay of rat serum and plasma converting enzyme. Hypertension. 1985 Mar-Apr;7 (2) :244-52.|[3]Horiuchi M, et al. Method for determination of angiotensin-converting enzyme activity in blood and tissue by high-performance liquid chromatography. J Chromatogr. 1982 Dec 10;233:123-30.