Biotinylated Human HLA-A*02:01&B2M&EBV LMP2 (FLYALALLL) Complex Protein (Monomer, MALS verified)

Epstein-Bar Virus (EBV), also known as human herpesvirus 4, belongs to gamma herpes virus family and is a very common human virus worldwide. EBV causes infectious mononucleosis (IM) and also associates to some specific types of cancers such as Burkitt’s lymphoma (BL) and gastric carcinoma (GC) . Glycoprotein B (gB) plays an important role in viral entry by binding with αvβ6/αvβ8 integrins to trigger the membrane fusion and entry process of epithelial cells, which makes it become an great target for EBV research. Epstein-Bar Virus (EBV), also known as human herpesvirus 4, belongs to gamma herpes virus family and is a very common human virus worldwide. EBV causes infectious mononucleosis (IM) and also associates to some specific types of cancers such as Burkitt’s lymphoma (BL) and gastric carcinoma (GC) . Glycoprotein B (gB) plays an important role in viral entry by binding with αvβ6/αvβ8 integrins to trigger the membrane fusion and entry process of epithelial cells, which makes it become an great target for EBV research. The Human HLA-A*0201 EBV LMP2 (FLYALALLL) complex protein is a complex of HLA-A*0201 of the MHC Class I, B2M and FLYALALLL peptide of the EBV LMP2.

Product Specifications

Background

Specifications

Biotinylated Human HLA-A*02:01&B2M&EBV LMP2 (FLYALALLL) Complex Protein is produced by co-expression of HLA and B2M loaded with EBV LMP2 peptide. This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™) . The protein has a calculated MW of 36.3 kDa and 11.7 kDa. The protein migrates as 40-43 kDa and 10 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

Accession Number

AAA59606.1 (HLA-A*02:01) & P61769-1 (B2M) & FLYALALLL

Expression Region

Gly 25 - Ile 308 (HLA-A*02:01) & Ile 21 - Met 119 (B2M) & FLYALALLL peptide

Host

HEK293

Target

HLA-A*0201 & B2M & EBV LMP2 (FLYALALLL)

Conjugation

Biotin-labeled

Tag

C-10xHis & C-Avi

Source

Human

Stability

-20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Endotoxin

1.0 EU per μg

Purity

90%

Bioactivity

Immobilized Biotinylated Human HLA-A*02:01&B2M&EBV LMP2 (FLYALALLL) Complex Protein (Cat. No. HL2-H82Er) at 1 μg/mL (100 μL/well) on streptavidin (Cat. No. STN-N5116) precoated (0.5 μg/well) plate can bind Anti-HLA class I Antibody, Human IgG1 (W6/32) with a linear range of 0.1-1.6 ng/mL (QC tested).

Format

Powder

Buffer

PBS, pH7.4

Additives

Trehalose

Molecular Weight

36.3 kDa and 11.7 kDa

Additionnal Information

Please see 'Shipping-and-Payments' sheet. Website: https://www.acrobiosystems.com/support/shipping-and-payments

Shipping Conditions

Storage Conditions

-20°C

Package Size

25ug*1

Host or Source

HEK293

Species

Human

Protein ID

AAA59606.1 (HLA-A*02:01) & P61769-1 (B2M) & FLYALALLL

Preservative

Trehalose

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