Anti-Superoxide dismutase (SOD) Mn Antibody

Rabbit Polyclonal Antibody specific to Superoxide dismutase (SOD) Mn

Product Specifications

CAS Number

9007-83-4

Product Name Alternative

EC 1.15.1.1

Gene Name

SOD2

NCBI Gene ID

UniProt

P04179

Accession Number

NP_000627.2

Cellular Locus

Mitochondrion matrix.

Host

Rabbit

Reactivity

Human, Monkey, Mouse, Rat, Bovine, Canine, Porcine, Hamster, Guinea Pig, Rabbit, Sheep, Chicken, Drosophila, Xenopus

Immunogen

Human Mn SOD

Target Antigen

Superoxide dismutase [Mn], mitochondrial

Target

Superoxide dismutase (SOD) Mn

Clonality

Polyclonal

Type

Antibody

Applications

WB, IHC, IP, ELISA

Field of Research

Enzymes

Purification Method

Purified by antigen immunoaffinity chromatography

Concentration

1.0 mg/mL

Dilution

Dilute in PBS or medium that is identical to that used in the assay system.

Format

Purified

Form

Liquid

Buffer

Phosphate Buffered Saline

Function

Destroys superoxide anion radicals which are normally produced within the cells and which are toxic to biological systems. {PubMed:10334867}.

Additionnal Information

Immunoblotting: use at 0.2-0.5ug/mL. Positive control: rat brain tissue extract Immunohistochemistry: 1-10ug/mL These are recommended concentrations. Endusers should determine optimal concentrations for their applications.

Storage Conditions

This antibody is stable for at least one (1) year at -20°C. Avoid multiple freeze-thaw cycles.

Specificity

This antibody detects a 25kDa protein on SDS-PAGE immunoblots corresponding to the molecular mass of Mn SOD. This antibody recognizes human, monkey, mouse, rat, bovine, canine, porcine, hamster, guinea pig, rabbit, sheep, chicken, Drosophila, and Xenopus Mn SOD.

Formulation

PBS, pH 7.0, 50% glycerol, and 0.09% sodium azide.

Buffer pH

pH 7.0

Target Background

Superoxide dismutase (SOD) is an endogenously produced intracellular enzyme that catalyzes the dismutation of the superoxide radical O2- to oxygen and hydrogen peroxide which are then metabolized to H2O and O2 by catalase and glutathione peroxidase. SODs play an important role in antioxidant defense mechanisms. Three different SOD isoenzymes are found in mammalian cells: SOD1, SOD2, and SOD3.