Anti-Beclin 2 Antibody

Rabbit Polyclonal Antibody specific to Beclin2

Product Specifications

CAS Number

9007-83-4

Product Name Alternative

Beclin-1 autophagy-related pseudogene 1, Beclin-1-like protein 1

Gene Name

BECN2

NCBI Gene ID

UniProt

A8MW95

Cellular Locus

Cytoplasm

Host

Rabbit

Reactivity

Human, Rat

Immunogen

Synthetic peptide corresponding to mid-protein amino acids of human Beclin 2.

Target Antigen

Beclin-2

Target

Beclin 2

Clonality

Polyclonal

Type

Antibody

Applications

WB, ICC/IF

Field of Research

Autophagy

Purification Method

Purified by Protein A affinity chromatography

Concentration

1.0 mg/mL

Dilution

Dilute in PBS or medium that is identical to that used in the assay system.

Format

Purified

Form

Liquid

Buffer

Phosphate Buffered Saline

Function

Involved in 2 distinct lysosomal degradation pathways: acts as a regulator of autophagy and as a regulator of G-protein coupled receptors turnover. Regulates degradation in lysosomes of a variety of G-protein coupled receptors via its interaction with GPRASP1/GASP1. {PubMed:23954414}.

Additionnal Information

Immunoblotting: use at 1-2ug/mL. A band of ~50kDa is detected.Detection of Beclin 2 in 20ug rat liver lysate. <br><br>These are recommended concentrations. <br><br>Endusers should determine optimal concentrations for their applications.

Storage Conditions

This antibody is stable for at least one (1) year at -20°C.

Specificity

This antibody recognizes human and rat Beclin 2.

Formulation

PBS, pH 7.4, 50% glycerol, 0.09% sodium azide.Concentration: 1mg/ml

Buffer pH

pH 7.4

Target Background

Autophagy is a catabolic process that results in the degradation of bulk cytoplasmic contents within autophagosomes and lysosomes. Beclin 2 is an Atg6/Beclin family member that functions in two distinct lysosomal degradation pathways, autophagy and the downregulation of certain seven- transmembrane-spanning GPCRs. Its role in GPCR lysosomal degradation does not involve Beclin 1 or other Beclin 1/2-interacting proteins such as ATG14 and VPS34 that function in autophagy. Its role in lysosomal degradation also appears to differ from other autophagy- independent functions previously described for yeast Atg6/Vps30 or mammalian Beclin 1, such as vacuolar protein sorting and EGFR degradation.