Biotinylated Human HLA-A*01:01&B2M Monomer Protein (Peptide free, MALS verified)

Biotinylated Human HLA-A*01:01&B2M Monomer Protein (HLM-H82Et) is expressed from CHO cells. It contains AA Ile 21 - Met 119 (B2M) & Gly 25 - Thr 305 (HLA-A*01:01) (Accession # P61769 (B2M) & Q5SUL5 (HLA-A*01:01) ) .

Product Specifications

Specifications

This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™) . The protein has a calculated MW of 48.9 kDa. The protein migrates as 33-43 kDa and 53-58 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

Accession Number

P61769 (B2M) & Q5SUL5 (HLA-A*01:01)

Expression Region

Ile 21 - Met 119 (B2M) & Gly 25 - Thr 305 (HLA-A*01:01)

Host

CHO

Target

HLA-A*0101 & B2M

Conjugation

Biotin-labeled

Tag

C-10xHis & C-Avi

Source

Human

Stability

-20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Endotoxin

1.0 EU per μg

Purity

90%

Bioactivity

Immobilized Biotinylated Human HLA-A*01:01&B2M Monomer Protein (Cat. No. HLM-H82Et) at 1 μg/mL (100 μL/well) on streptavidin (Cat. No. STN-N5116) precoated (0.5 μg/well) plate can bind Anti-HLA class I Antibody, Human IgG1 (W6/32) with a linear range of 0.1-1.6 ng/mL (QC tested).