Rat anti EpCAM / CD326

Ep-CAM is a 40 kD glycoprotein and can be detected at the basolateral membrane of the majority of epithelial tissues, where it is intriCately linked with the cadherin-Catenin complex and hence the fundamental WNT pathway responsible for intracellular signalling and polarity. This antigen functions as a homotypic calcium-independent cell adhesion molecule. Of particular interest, Ep-CAM appears to be overexpressed by the majority of Human epithelial carcinomas, including colorectal, breast, prostate, head and neck, and hepatic carcinomas. The antigen is being used as a target for immunotherapy of Human carcinomas. Formation of Ep-CAM-mediated adhesions has a negative regulatory effect on adhesions mediated by classic cadherins, which may have strong effects on the differentiation and growth of epithelial cells. Ep-CAM overexpression was suggested to be associated with enhanced epithelial prolifeRation.

G8.8 is a rat monoclonal IgG2a antibody derived by fusion of X63-Ag8.653 mouse myeloma cells with spleen lymphocytes from rats repeatedly immunized with glycoconjugates from BALB/c Mouse derived medullary thymic epithelial cells.

G8.8 is suitable for immunoblotting, immunoprecipitation, immunocytochemistry and immuno-histochemistry on frozen tissues and flow cytometry. Optimal antibody dilution should be determined by titration; recommended range is 1:25 – 1:200 for flow cytometry, and for immunohistochemistry with avidin-biotinylated Horseradish peroxidase complex (ABC) as detection reagent, and 1:100 – 1:1000 for immunoblotting applications.

This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving Humans or animals. This product contains sodium azide. To prevent formation of toxic vapors, do not mix with strong acidic solutions. To prevent formation of potentially explosive metallic azides in metal plumbing, always wash into drain with copious quantities of water. This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.

1. Farr, A., Nelson, A., Truex, J. And Hosier, S. (1991). Epithelial heterogeneity in the murine thymus: a cell surface glycoprotein expressed by subcapsular and medullary epithelium. The Journal of Histochemistry and Cytochemistry 39, 645-53. _x000D_ 2. Lee, M., Sharrow, S., Farr, A., Singer, A. and Udey M. (1994). Expression of the homotypic adhesion molecule E-cadherin by immature murine thymocytes and thymic epithelial cells. Journal of Immunology Mol 152, 5653-59. _x000D_ 3. Borkowski, T., Nelson, A., Farr, A. and Udey, M. (1996). Expression of gp40, the murine homologue of Human epithelial cell adhesion molecule (Ep-Cam), by murine dendritic cells. Eur. J. Immunol. 26, 110-14. _x000D_ 4. Nelson, A., Dunn, R., Peach, R., Aruffo, A. and Farr, A. (1996). The murine homolog of Human Ep-CAM, a homotypic adhesion molecule, is expressed by thymocytes and thymic epithelial cells. Eur. J. Immunol. 26, 401-408. _x000D_ 5. Went, P., Vasei, M., Bubendorf, L., Terracciano, L., Tornillo, L., Riede, U., Kononen, J., Simon, R., Sauter, G. And Baeuerle, P.A. (2006). Frequent high-level expression of the immunotherapeutic target Ep-CAM in colon, stomach, prostate and lung cancers bladder. British Journal of Cancer 94, 128-135. _x000D_ 6. Maaser, K. and Borlak, J. (2008). A genom-wide expression analysis identifies a network of EpCAM-induced cel cycle regulators. British Journal of Cancer 99, 1635-43

The antibody is shipped at ambient temperature and may be stored at +4°C; For prolonged storage prepare appropriate aliquots and store at or below -20°C; Prior to use, an aliquot is thawed slowly in the dark at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2); Repeated thawing and freezing should be avoided; Working dilutions should be stored at +4°C, not refrozen, and preferably used the same day; If a slight precipitation occurs upon storage, this should be removed by centrifugation; It will not affect the performance or the concentration of the product