Anti-Beclin 1 Antibody

Rabbit Polyclonal Antibody specific to Beclin1

Product Specifications

CAS Number

9007-83-4

Product Name Alternative

Coiled-coil myosin-like BCL2-interacting protein, Protein GT197 [Cleaved into: Beclin-1-C 35 kDa; Beclin-1-C 37 kDa]

Gene Name

BECN1

NCBI Gene ID

UniProt

Q14457

Cellular Locus

Cytoplasm, Golgi apparatus, trans-Golgi network membrane, Endosome membrane, Endoplasmic reticulum membrane, Mitochondrion membrane, Endosome, Cytoplasmic vesicle, autophagosome

Host

Rabbit

Reactivity

Human, Rat

Immunogen

Synthetic peptide corresponding to mid-protein amino acids of human Beclin 1.

Target Antigen

Beclin-1

Target

Beclin 1

Clonality

Polyclonal

Type

Antibody

Applications

WB, ICC/IF

Field of Research

Autophagy

Purification Method

Purified by Protein A affinity chromatography

Concentration

1.0 mg/mL

Dilution

Dilute in PBS or medium that is identical to that used in the assay system.

Format

Purified

Form

Liquid

Buffer

Phosphate Buffered Saline

Function

Plays a central role in autophagy (PubMed:18570871, PubMed:21358617, PubMed:23184933, PubMed:23974797, PubMed:28445460). Acts as core subunit of the PI3K complex that mediates formation of phosphatidylinositol 3-phosphate; different complex forms are believed to play a role in multiple membrane trafficking pathways: PI3KC3-C1 is involved in initiation of autophagosomes and PI3KC3-C2 in maturation of autophagosomes and endocytosis. Involved in regulation of degradative endocytic trafficking and required for the abcission step in cytokinesis, probably in the context of PI3KC3-C2 (PubMed:20643123, PubMed:20208530, PubMed:23974797, PubMed:26783301). Essential for the formation of PI3KC3-C2 but not PI3KC3-C1 PI3K complex forms. Involved in endocytosis (PubMed:25275521). Protects against infection by a neurovirulent strain of Sindbis virus (PubMed:9765397). May play a role in antiviral host defense. {PubMed:18570871, PubMed:20208530, PubMed:20643123, PubMed:21358617, PubMed:23184933, PubMed:23974797, PubMed:25275521, PubMed:26783301, PubMed:28445460, PubMed:9765397, PubMed:21364619, PubMed:26263979}.

Additionnal Information

Immunoblotting: use at 1- 2ug/mL. A band of ~51kDa is detected. Immunofluorescence: use at 10ug/mL. These are recommended concentrations. Endusers should determine optimal concentrations for their applications.

Storage Conditions

This antibody is stable for at least one (1) year at -20°C.

Specificity

This antibody recognizes human and rat Beclin 1.

Formulation

PBS, pH 7.4, 50% glycerol, 0.09% sodium azide.Concentration: 1mg/ml

Buffer pH

pH 7.4

Target Background

Autophagy is a catabolic process that results in the degradation of bulk cytoplasmic contents within autophagosomes and lysosomes. Beclin 1, the mammalian orthologue of yeast Atg6, has a central role in autophagy. It interacts with several cofactors (Atg14L, UVRAG, Bif-1, Rubicon, Ambra1, HMGB1, nPIST, VMP1, SLAM, IP3R, PINK and survivin) to regulate the lipid kinase Vps-34 protein and promote formation of Beclin 1-Vps34-Vps15 core complexes, thereby inducing autophagy. In contrast, the BH3 domain of Beclin 1 is bound to, and inhibited by Bcl-2 or Bcl-XL. This interaction can be disrupted by phosphorylation of Bcl-2 and Beclin 1, or ubiquitination of Beclin 1. Beclin 1 dysfunction has been implicated in many disorders including cancer and neurodegeneration.