3-Acetylpyridine adenine dinucleotide

3-Acetylpyridine adenine dinucleotide functions as an alternative reductant in glutamate-synthase-catalyzed glutamate synthesis. 3-Acetylpyridine adenine dinucleotide demonstrates kinetic parameters in the AcPdADH-dependent reaction wherein Kcat and Km values for other substrates are reduced approximately fourfold relative to the NADH-dependent reaction. 3-Acetylpyridine adenine dinucleotide shows a Km of ~3 µM and acts as a competitive inhibitor of NADH in NADH-dependent glutamate synthesis with a Ki of 1 µM. 3-Acetylpyridine adenine dinucleotide provides meaningful mechanistic insights into redox cofactor selectivity in enzymatic glutamate biosynthesis.