Fluo-3FF AM

Fluo-3FF AM is a low-affinity (Kd = 42 μM), Mg 2+ insensitive, and relatively photostable fluorescent Ca 2+ sensitive indicator (Abs/Em = 462 nm/526 nm) . It is essentially non-fluorescent but exhibits strong fluorescence enhancement upon entry into cells and binding to calcium. [1]

ClC=1C=C2C(=C3C(OC2=CC1OCOC(C)=O)=CC(=O)C(Cl)=C3)C4=CC(OCCOC5=C(N(CC(OCOC(C)=O)=O)CC(OCOC(C)=O)=O)C=CC(F)=C5F)=C(N(CC(OCOC(C)=O)=O)CC(OCOC(C)=O)=O)C=C4