Mca-AVLQSGFR-K (Dnp) -K-NH2 TFA

Mca-AVLQSGFR-K (Dnp) -K-NH2 serves as a fluorogenic substrate specifically designed for the main protease (Mpro) of the severe acute respiratory syndrome coronavirus (SARS-CoV), also referred to as 3C-like protease (3CLpro) . This substrate, upon enzymatic cleavage by SARS-CoV Mpro, liberates 7-methoxycoumarin-4-acetyl (Mca) which emits fluorescence. This fluorescence emission, with excitation/emission peaks at 328/420 nm respectively, facilitates the quantification of SARS-CoV Mpro's catalytic activity.

NCCCC[C@@H](C(N)=O)NC([C@H](CCCCNC1=C(C=C(C=C1)[N+]([O-])=O)[N+]([O-])=O)NC([C@H](CCCNC(N)=N)NC([C@H](CC2=CC=CC=C2)NC(CNC([C@H](CO)NC([C@H](CCC(N)=O)NC([C@H](CC(C)C)NC([C@H](C(C)C)NC([C@H](C)NC(CC(C3=CC=C(OC)C=C3O4)=CC4=O)=O)=O)=O)=O)=O)=O)=O)=O)=O)=O.OC(C(F)(F)F)=O