DC432 TFA

DC432 is a cell-permeable peptide that serves as an inhibitor of N-terminal Xaa-Pro-Lys N-methyltransferase 1 (NTMT1; IC50 = 0.054 μM), capable of binding to both NTMT1 and NTMT2 with dissociation constants (Kds) of 0.3 and 1 μM, respectively. It effectively inhibits the N-terminal dimethylation of regulator of chromosome condensation 1 (RCC1) by NTMT1 at concentrations of 10 and 50 μM in cell-free assays. Furthermore, at a concentration of 100 μM, DC432 diminishes the trimethylation of the RCC1 SPKRIA motif in HCT116 cells.

N=C(N)NCCC[C@@H](C(N)=O)NC([C@H](CCCNC(N)=N)NC([C@H](CCCNC(N)=N)NC([C@H](CCCNC(N)=N)NC([C@H](CCCNC(N)=N)NC([C@H](CCCNC(N)=N)NC([C@H](CCCCN)NC([C@H]1N(CCC1)C(CC2=CC=C(C=C2)O)=O)=O)=O)=O)=O)=O)=O)=O.OC(C(F)(F)F)=O