RE (EDANS) EVNLDAEFK (DABCYL) R

RE (EDANS) EVNLDAEFK (DABCYL) R is a double-labeled peptide with EDANS and DABCYL, serving as a fluorescent substrate for BACE1 with an excitation wavelength of 360 nm and an emission wavelength of 528 nm. It is used to quantify BACE1 activity, with fluorescence intensity proportional to enzyme activity levels [1].

N(CCNC(CC[C@@H](C(N[C@H](C(N[C@H](C(N[C@H](C(N[C@H](C(N[C@H](C(N[C@H](C(N[C@H](C(N[C@@H](CC1=CC=CC=C1)C(N[C@H](C(N[C@@H](CCCNC(=N)N)C(O)=O)=O)CCCCNC(=O)C2=CC=C(N=NC3=CC=C(N(C)C)C=C3)C=C2)=O)=O)CCC(O)=O)=O)C)=O)CC(O)=O)=O)CC(C)C)=O)CC(N)=O)=O)C(C)C)=O)CCC(O)=O)=O)NC([C@H](CCCNC(=N)N)N)=O)=O)C=4C5=C(C(S(=O)(=O)O)=CC=C5)C=CC4